亚洲国产美女精品久久,无码人妻在线视频

鄭重說明:

您值得信賴的中藥成分及標準合作伙伴滿足您研發和工業化需求

產品關鍵詞：香草酸-4-β-D-葡萄糖苷 6-甲氧基山奈酚茯苓新酸B 去甲異波爾定

guanyuwomen關于德思特

聯系方式

服務熱線：

028-8500-3950

公司傳真：	028-85589956
公司郵箱：	sales@tcmlmt.com
公司網址：	www.hamilton.org.cn
公司地址：	四川省成都市溫江區海科路西段128號5棟

發表文獻展示

當前位置:首頁關于德思特 > 發表文獻展示 >

Fitoterapia-15 July 2019 穿心蓮黃酮苷E

時間：2020-09-03 瀏覽次數：分享到：

Screening cyclooxygenase-2 inhibitors from Andrographis paniculata to treat inflammation based on bio-affinity ultrafiltration coupled with UPLC-QTOF-MS
期刊名：Fitoterapia
文獻編號：DOI 10.1016/j.fitote.2019.104259
文獻地址：https://www.sciencedirect.com/science/article/abs/pii/S0367326X19309633?via%3Dihub
發表日期：15 July 2019

Abstract
The cyclooxygenase-2 (COX-2) is a key enzyme in the synthesis of prostaglandins, its inhibitors are effective for the treatment of inflammation. In this study, an analytical method based on bio-affinity ultrafiltration coupled with ultra performance liquid chromatography and quadrupole-time-of-flight mass spectrometry (BAUF-UPLC-Q-TOF-MS) was established for rapidly screening and identifying COX-2 ligands. Meanwhile, the specificity of the method was verified by denatured COX-2 and inactive compound. Next, the biological activity of ligands screened was proved by enzyme inhibition assay. The preferred binding modes for these COX-2 inhibitors were then determined by molecular docking. Finally, network pharmacology was used to explore the pathways involved anti-inflammatory effects. As a result, five COX-2 inhibitors were selected in the extract of Andrographis Herba (AH), including andrographolide (1), 14-deoxy-11,12-didehydroandrographiside (2), andrographidine E (3), andrographidine D (4), and deoxyandrographolide (5). Among them, compounds 2, 3, 4 and 5 were reported to have COX-2 inhibitory activity for the first time. The result of COX-2 inhibition assay showed that compound 3 had an IC50 of 19?μM, compounds 2 and 5 had an IC50 of >200?μM. And each ligand could bind to multiple amino acid residues of COX-2 based on molecular docking. At last, combined with network pharmacology, these ligands could exert anti-inflammatory effects through three pathways related to COX-2, arachidonic acid metabolism, synthesis of prostaglandins, and prostanoid ligand receptors. The method established in the study could be used to rapidly screen other enzyme inhibitors in complex mixtures, and help to understand the mechanism of action.

14-deoxy-11,12-didehydroandrographiside, andrographidine E and deoxyandrographolide were purchased from Chengdu DeSiTe Biological Technology Co., Ltd. (Chengdu, China).

上一篇：Journal of Pharmaceutical and Biomedical Analysis-10 August 2018

下一篇：Frontiers in pharmacology-09 January 2019 異槲皮苷穗花杉雙黃

Afrormosine CAS:550-79-8 中藥對照品標準品

產品編碼：DA0040產品名稱：Afrormosine英文名:AfrormosineCAS登錄...

人妻少妇免费午夜无码区,久久爆乳AⅤ无码一区二区,日本精品久久久久影院日本,99精品66AV99精品