天然藥物活性單體及中藥對照品專業生產供應商
聯系方式
028-85003950
天然藥物活性單體及中藥對照品專業生產供應商
028-85003950
您值得信賴的中藥成分及標準合作伙伴 滿足您研發和工業化需求
產品關鍵詞： 香草酸-4-β-D-葡萄糖苷 6-甲氧基山奈酚 茯苓新酸B 去甲異波爾定
028-8500-3950
| 公司傳真： | 028-85589956 |
| 公司郵箱： | sales@tcmlmt.com |
| 公司網址： | www.hamilton.org.cn |
| 公司地址： | 四川省成都市溫江區海科路西段128號5棟 |
Rhubarb–Radix scutellariae is a classic herb pair, which is commonly used to clear away heat and toxin in clinic. The aim of this study was to investigate the influence of compatibility of Rhubarb and Radix scutellariae on the pharmacokinetic behaviors of anthraquinones and flavonoids in rat plasma.
Eighteen rats were randomly divided into three groups, and were orally administered Rhubarb and/or Radix scutellariae extracts. A sensitive and rapid UPLC–MS/MS method was developed and validated to determine the concentrations of baicalin, baicalein, wogonside, wogonin, rhein, and emodin in rat plasma. The concentrations of phase II conjugates of flavonoid aglycones and anthraquinone aglycones were also determined after hydrolyzing the plasma with sulfatase.
Compared with administration of Radix scutellariae alone, co-administration of Rhubarb significantly decreased the first maximum plasma concentration (C max1) of baicalin, wogonside, and the phase II conjugates of baicalein, wogonin to 46.40, 61.27, 41.49, and 20.50%, respectively. The area under the plasma concentration–time curve from time zero to infinity (AUC0–∞) was significantly decreased from 82.60 ± 20.22 to 51.91 ± 7.46 μM·h for rhein and 276.83 ± 98.02 to 175.42 ± 86.82 μM·h for the phase II conjugates of wogonin after compatibility. The time to reach the first maximum plasma concentration (T max1) of anthraquinones was shortened and the second peak of anthraquinones disappeared after compatibility.
Compatibility of Rhubarb and Radix scutellariae can significantly affect the pharmacokinetic behaviors of characteristic constituents of the two herbs. The cause of these pharmacokinetic differences was further discussed combined with the in vivo ADME (absorption, disposition, metabolism, and excretion) processes of anthraquinones and flavonoids.
Rhein (R), emodin (E), baicalin (BG), baicalein (B), wogonside (WG), and wogonin (W) standards were purchased from Chengdu Desite Biological Technology Co., Ltd. (Sichuan, China).
