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采用旋渦輔助固相分散法同時提取防風藤中5種有效成分

時間:2022-03-14   瀏覽次數:  分享到:
原文鏈接:https://doi.org/10.1016/j.scp.2021.100524

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Abstract:

Saposhnikoviae Radix (SR), one of top-grade herbs, is widely used to treat cold, headache, fevers, cough, rheumatism and cardiovascular diseases in prescriptions. A vortex-assisted matrix solid phase dispersion (VA-MSPD) extraction method applying a reusable dispersant was developed to extract four chromones and one coumarin from Saposhnikoviae Radix (SR), which followed by high performance liquid chromatography analysis (HPLC). The novel dispersant, acetonitrile modified carbon-coated magnetite nanoparticles (Fe3O4@C@ ACN MNP), could be reused for 10 times and it still possesses excellent extraction performance. Various optimal experimental condition of VA-MSPD was considered and obtained reliable matrix linearity (r2 > 0.999) and good recovery (94.8%–105%). In order to recovery the dispersant from sample-dispersant powder after elution, methanol/water (50:50, v/v) was applied as washing solution to separate the dispersant and samples, so that the percent recovery of modified materials was obtained as 88.6% (RSD = 0.74%). The results indicated that Fe3O4@C@ACN MNP could be considered as sustainable dispersant and it exhibited tremendous potential for efficacious extraction of chromones and coumarins.

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2. Materials and methods 

 2.1. Chemicals and reagents  

   Reference components of 4-O-β-D-glucosyl-5-O-methylvisamminol,  prim-O-D-glucosylcimifugin, cimifugin, psoralen and sec-O-glucosylha-maudol (purity >99%) were supplied by Chengdu Desite Biotech Co.,  Ltd. (Chengdu, China). 

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